Journal: Journal of virological methods

Article Title: A broad-based probe-free qPCR assay for detection and discrimination of three human herpes viruses

doi: 10.1016/j.jviromet.2023.114824

Figure Lengend Snippet: Comparison of Phusion vs Q5 DNA Polymerases for amplification of HSV1. Amplification of serially diluted HSV1 (5.23 × 10 5 to 5.23 ×10 2 copies/reaction) in triplicates with (A) Phusion-LOD of 5.23 × 10 4 copies and with (B) Q5-LOD of 5.23 × 10 3 copies.

Article Snippet: Extracted viral DNA was obtained from ATCC with the following product details: HCMV strain AD-169-ATCC VR-538D, HSV1 strain KOS-ATCC VR-1493D and HSV2 strain G-ATCC VR-734.

Techniques: Comparison, Amplification

Journal: Journal of virological methods

Article Title: A broad-based probe-free qPCR assay for detection and discrimination of three human herpes viruses

doi: 10.1016/j.jviromet.2023.114824

Figure Lengend Snippet: Optimization of annealing temperature in HSV1. Temperature gradient experiments were performed for HSV1 in duplicates from 72 °C to 67 °C (rounded off) for a 10× dilution series ranging from 5.23×10 4 to 5.23 × 10 2 genome copies/reaction. As in the case of HCMV, product concentration decreases with decrease in temperature. ~72 °C was thus chosen as the optimum annealing temperature.

Article Snippet: Extracted viral DNA was obtained from ATCC with the following product details: HCMV strain AD-169-ATCC VR-538D, HSV1 strain KOS-ATCC VR-1493D and HSV2 strain G-ATCC VR-734.

Techniques: Concentration Assay

Journal: Journal of virological methods

Article Title: A broad-based probe-free qPCR assay for detection and discrimination of three human herpes viruses

doi: 10.1016/j.jviromet.2023.114824

Figure Lengend Snippet: Standard curves for HCMV, HSV1 and HSV2. The quantification region (LOQ) is marked with a dashed line while the plateau region at lower concentrations is due to primer dimers. The serial dilution concentrations are as follows: HCMV-8.45 × 10 7 to 8.45×10 0 copies/reaction, HSV1– 5.23 × 10 7 to 5.23 × 10 0 copies/reaction and HSV2– 3.45 × 10 6 to 3.45 × 10 0 copies/reaction.

Article Snippet: Extracted viral DNA was obtained from ATCC with the following product details: HCMV strain AD-169-ATCC VR-538D, HSV1 strain KOS-ATCC VR-1493D and HSV2 strain G-ATCC VR-734.

Techniques: Serial Dilution

Journal: Journal of virological methods

Article Title: A broad-based probe-free qPCR assay for detection and discrimination of three human herpes viruses

doi: 10.1016/j.jviromet.2023.114824

Figure Lengend Snippet: Characteristics of the viral assay for HCMV, HSV1 and HSV2 amplification.

Article Snippet: Extracted viral DNA was obtained from ATCC with the following product details: HCMV strain AD-169-ATCC VR-538D, HSV1 strain KOS-ATCC VR-1493D and HSV2 strain G-ATCC VR-734.

Techniques: Amplification

Journal: Journal of virological methods

Article Title: A broad-based probe-free qPCR assay for detection and discrimination of three human herpes viruses

doi: 10.1016/j.jviromet.2023.114824

Figure Lengend Snippet: Inter and intra-assay variation for the three viruses.

Article Snippet: Extracted viral DNA was obtained from ATCC with the following product details: HCMV strain AD-169-ATCC VR-538D, HSV1 strain KOS-ATCC VR-1493D and HSV2 strain G-ATCC VR-734.

Techniques: Intra Assay, Inter Assay

Journal: Gene Therapy

Article Title: An overview of development in gene therapeutics in China

doi: 10.1038/s41434-020-0163-7

Figure Lengend Snippet: Ongoing clinical trials of gene therapy in China.

Article Snippet: 11 , OrienGene Biotechnology Ltd. , OrienX010 (recombinant human GM-CSF HSV-1) , Tumor site local injections , NA , Melanoma , Phase I , NCT03048253 , 2017 , Unknown.

Techniques: Clinical Proteomics, Injection, Genetically Modified, Plasmid Preparation, CRISPR, Knock-Out, Recombinant, Virus